Iran live updates: Trump says he thinks Iran agreement is 'over' after exchange of fire

Lead Iranian negotiator Mohammad Bagher Ghalibaf, accused the U.S. of "major MOU violations" after U.S. airstrikes on Tuesday.

In a statement on social media, Ghalibaf, who also serves as Iran's Parliament speaker, listed a number of items he claimed as violations of the memorandum of understanding between Iran and the U.S. that temporarily paused hostilities.

Among them: "Violating Iranian adjustments in the Strait"; "Persistent threats of further strikes"; and "Reinstating oil sanctions."

The post ended: "The era of bullying and extortion is over. It leads nowhere. We don’t fold."

The U.S. said it carried out the retaliatory airstrikes in response to Iranian attacks on commercial vessels traveling through the Strait of Hormuz.

U.S. forces hit over 80 targets overnight in a new round of airstrikes that came as an "immediate response" to Iran's attacks on three commercial vessels transiting the Strait of Hormuz, U.S. Central Command said.

"U.S. forces struck Iranian air defense systems, command and control networks, coastal radar sites, anti-ship missile capabilities, and more than 60 Islamic Revolutionary Guard Corps small boats in and near the strait to degrade Iran’s ability to continue attacking international commerce flowing through the international trade corridor," CENTCOM said in a social media post.

CENTCOM described them as "offensive strikes" -- in contrast to strikes last month that the U.S. military characterized as "self-defense strikes."

CENTCOM said the recent strikes targeting commercial tankers in the strait amounted to "unwarranted aggression" and a clear and dangerous violation of the ceasefire and undermines freedom of navigation.

"CENTCOM forces remain postured and prepared to hold Iran accountable when the agreement is not adhered to or obeyed," the U.S. statement said.

Tonight's airstrikes on Iran targeted port facilities, missile and drone sites, coastal surveillance sites and air defense sites, according to two U.S. officials.

The strikes are expected to be much larger in scope and scale than the U.S. attacks on Iran in late June, according to a U.S. official.

Last month's strikes were also launched in response to Iranian aggression in the Strait of Hormuz, but officials said that more limited military action then was designed to avoid upending the memorandum of understanding with Iran.

-ABC News' Shannon Kingston and Luis Martinez

Iran's deputy foreign minister, in a statement published by the FARS news agency, is calling the U.S. strikes on Iran a serious violation of the memorandum of understanding signed last month between the U.S. and Iran.

"Iran will take decisive measures to protect its national interests and security, while issuing a serious warning about the consequences of America's breach of the treaty," the deputy foreign minister said in the statement.

Iranian officials also slammed the U.S. government's decision to revoke a license that authorized the sale of Iranian oil -- a key aspect of the MOU -- following Iran's reported missile strikes on tankers in the Strait of Hormuz.